Analysis Note

ControlSK-N-SH cell lysate

Application

Immunohistochemistry Analysis: Human brain tissue was prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using 2.5 µg/mL Slitrk3 antibody.

Research CategoryNeuroscienceNeuroscience

Research Sub CategoryDevelopmental NeuroscienceNeurofilament & Neuron Metabolism

This Slitrk3 antibody is validated for use in WB & IHC for the detection of the Slitrk3 protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SLIT and NTRK-like family 3 (Slitrk3) is a member a protein family consisting of six homologous transmembrane proteins (Slitrk1-6) that share two conserved leucine-rich repeat domains in the extracellular domain and have significant homology to Slit, a secreted axonal growth-controlling protein. These proteins are also homologous to trk neurotrophin receptors in their intracellular domains. Expression of Slitrk proteins is highly restricted to neural and brain tumor tissues, but varies within the protein family. Overexpression of Slitrk3 inhibited neurite outgrowth in cultured neurons, suggesting that these proteins are involved in the control of neurite outgrowth.

Immunogen

KLH-conjugated linear peptide corresponding to human Slitrk3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blot in SK-N-SH cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Slitrk3 in 15 µg of SK-N-SH cell lysate.

Specificity

This antibody is predicted to have no cross-reactivity to other Slitrk proteins.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~120 kDa observed